This manuscript describes the establishment of an infectious model of pneumonia in mice and the respective characterization of injury resolution along with methods for growing bacteria and intratracheal instillation. A novel approach using high-dimensional flow cytometry to evaluate the immune landscape is also described.
Villabona-Rueda, A., Wang, D., D’Alessio, F. R. Experimental Model to Evaluate Resolution of Pneumonia. J. Vis. Exp. (192), e63925, doi:10.3791/63925 (2023).