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Experimental Model to Evaluate Resolution of Pneumonia
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Experimental Model to Evaluate Resolution of Pneumonia

Experimental Model to Evaluate Resolution of Pneumonia

DOI:
10.3791/63925-v

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09:49 min

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February 17, 2023

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, ,
1Division of Pulmonary Critical Care Medicine,Johns Hopkins University School of Medicine, 2Engineering Management, Whiting School of Engineering,Johns Hopkins Üniversitesi

Bölümler

  • 00:00Introduction
  • 01:11Bacteria Thawing for Intratracheal Instillation
  • 02:00Intratracheal Instillation of Live Bacteria
  • 03:44Bronchoalveolar Lavage (BAL) and Lung Harvest
  • 05:55Bronchoalveolar Lavage Processing
  • 06:38Lung Processing for Single Cell Suspension
  • 08:01Results: Experimental Model to Evaluate Resolution of Pneumonia
  • 09:29Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This manuscript describes the establishment of an infectious model of pneumonia in mice and the respective characterization of injury resolution along with methods for growing bacteria and intratracheal instillation. A novel approach using high-dimensional flow cytometry to evaluate the immune landscape is also described.

Etiketler

PneumoniaARDSAcute Lung InjuryStreptococcus PneumoniaeKlebsiella PneumoniaeC57BL6 MiceBronchoalveolar LavageLung Injury MarkersCell CountBacterial Colony-forming UnitsHistologyHigh Dimensional Flow Cytometry

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