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Immunology and Infection
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Experimental Model to Evaluate Resolution of Pneumonia
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Experimental Model to Evaluate Resolution of Pneumonia

Experimental Model to Evaluate Resolution of Pneumonia

English

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805 Views

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09:49 min

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February 17, 2023

DOI:

10.3791/63925-v

DOI:
10.3791/63925-v

•

09:49 min
February 17, 2023

•

6 Views
, ,
1Division of Pulmonary Critical Care Medicine,Johns Hopkins University School of Medicine, 2Engineering Management, Whiting School of Engineering,Johns Hopkins University

Summary

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This manuscript describes the establishment of an infectious model of pneumonia in mice and the respective characterization of injury resolution along with methods for growing bacteria and intratracheal instillation. A novel approach using high-dimensional flow cytometry to evaluate the immune landscape is also described.

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Cite this Article

Villabona-Rueda, A., Wang, D., D’Alessio, F. R. Experimental Model to Evaluate Resolution of Pneumonia. J. Vis. Exp. (192), e63925, doi:10.3791/63925 (2023).

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