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Isolation of Preadipocytes from Broiler Chick Embryos
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Isolation of Preadipocytes from Broiler Chick Embryos

Isolation of Preadipocytes from Broiler Chick Embryos

DOI:
10.3791/63861-v

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06:32 min

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August 04, 2022

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, , , ,
1Department of Animal Science,University of Tennessee

Chapters

  • 00:05Introduction
  • 01:03Adipose Tissue Collection and Digestion
  • 02:21Digestion of Adipose Tissue
  • 04:48Results: Comparison of Cell Morphology and Lipid Accumulation
  • 05:47Conclusion

Summary

자동 번역

자동 번역

The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.

Tags

Preadipocyte IsolationBroiler Chick EmbryosAdipocyte DifferentiationPrimary PreadipocytesEmbryonic Fat GrowthPoultry Research Model

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