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August 04, 2022
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The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.
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Kim, M., Jung, U., Shepherd, E., Mihelic, R., Voy, B. H. Isolation of Preadipocytes from Broiler Chick Embryos. J. Vis. Exp. (186), e63861, doi:10.3791/63861 (2022).
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