Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Isolation of Preadipocytes from Broiler Chick Embryos
2,009 Views
•
06:32 min
•
August 04, 2022
DOI:
Summary
Automatically generated
The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.
Read Article
Cite this Article
Kim, M., Jung, U., Shepherd, E., Mihelic, R., Voy, B. H. Isolation of Preadipocytes from Broiler Chick Embryos. J. Vis. Exp. (186), e63861, doi:10.3791/63861 (2022).
Copy