Journal
/
생화학
/
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

DOI:
10.3791/63720-v

•

09:20 min

•

April 11, 2022

•

, , , ,
1Department of Chemistry,University of Colorado, Denver, 2Department of Chemistry & Biochemistry,University of Denver, 3Analytical Resources Core Bioanalysis & Omics,Colorado State University

Chapters

  • 00:09Introduction
  • 01:05Concentration Determination of RNA Solution
  • 03:15Hydrolysis of Oligonucleotides of RNA by Xrn-1
  • 05:31Desalting of RNA Solution, MALDI-TOF Plate Spotting
  • 07:41Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry
  • 08:32Conclusion

Summary

자동 번역

자동 번역

MALDI-TOF was used to characterize fragments obtained from the reactivity between oxidized RNA and the exoribonuclease Xrn-1. The present protocol describes a methodology that can be applied to other processes involving RNA and/or DNA.

Tags

RNA FragmentsEnzymatic DegradationMALDI-TOF Mass SpectrometryBiopolymer CharacterizationBiochemical TransformationsUV-Vis SpectrophotometryPeltier Temperature Control

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Read Article