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Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
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Biochemistry
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JoVE Journal Biochemistry
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

English

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3,077 Views

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09:20 min

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April 11, 2022

DOI:

10.3791/63720-v

DOI:
10.3791/63720-v

•

09:20 min
April 11, 2022

•

3 Views
, , , ,
1Department of Chemistry,University of Colorado, Denver, 2Department of Chemistry & Biochemistry,University of Denver, 3Analytical Resources Core Bioanalysis & Omics,Colorado State University

Summary

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MALDI-TOF was used to characterize fragments obtained from the reactivity between oxidized RNA and the exoribonuclease Xrn-1. The present protocol describes a methodology that can be applied to other processes involving RNA and/or DNA.

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Schowe, S. W., Langeberg, C. J., Chapman, E. G., Brown, K., Resendiz, M. J. E. Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry. J. Vis. Exp. (182), e63720, doi:10.3791/63720 (2022).

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