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Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
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生物化学
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JoVE 杂志 生物化学
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

DOI:
10.3791/63720-v

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09:20 min

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April 11, 2022

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, , , ,
1Department of Chemistry,University of Colorado, Denver, 2Department of Chemistry & Biochemistry,University of Denver, 3Analytical Resources Core Bioanalysis & Omics,Colorado State University

Chapters

  • 00:09Introduction
  • 01:05Concentration Determination of RNA Solution
  • 03:15Hydrolysis of Oligonucleotides of RNA by Xrn-1
  • 05:31Desalting of RNA Solution, MALDI-TOF Plate Spotting
  • 07:41Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry
  • 08:32Conclusion

Summary

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MALDI-TOF was used to characterize fragments obtained from the reactivity between oxidized RNA and the exoribonuclease Xrn-1. The present protocol describes a methodology that can be applied to other processes involving RNA and/or DNA.

Tags

RNA FragmentsEnzymatic DegradationMALDI-TOF Mass SpectrometryBiopolymer CharacterizationBiochemical TransformationsUV-Vis SpectrophotometryPeltier Temperature Control

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