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The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
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The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

2,062 Views

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08:37 min

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November 02, 2021

DOI:

10.3791/63242-v

DOI:
10.3791/63242-v

•

08:37 min
November 02, 2021

•

1 Views
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1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,The University of Melbourne

Summary

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Open searching enables the identification of glycopeptides decorated with previously unknown glycan compositions. Within this article, a streamlined approach for undertaking open searching and subsequent glycan-focused glycopeptide searches are presented for bacterial samples using Acinetobacter baumannii as a model.

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Acinetobacter BaumanniiO-linked GlycopeptidesOpen SearchingGlycosylationProteomeSTB-RPSZIC-HILICChromatographyEnrichmentMass Spectrometry

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Cite this Article

Lewis, J. M., Coulon, P. M. L., McDaniels, T. A., Scott, N. E. The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides. J. Vis. Exp. (177), e63242, doi:10.3791/63242 (2021).

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