Journal
/
Bioquímica
/
The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

DOI:
10.3791/63242-v

•

08:37 min

•

November 02, 2021

•

, , ,
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,The University of Melbourne

Capítulos

  • 00:04Introduction
  • 00:44Processing of Proteome Samples
  • 04:01Liquid Chromatography-Mass Spectrometry (LC-MS) of Proteome/Glycopeptide-Enriched Samples
  • 04:28Analysis of Proteome/Glycopeptide-Enriched Samples
  • 06:08Results: Open Searching and Glycan-Focused Glycopeptide Searches for Acinetobacter baumannii
  • 07:52Conclusion

Summary

Traducción Automática

Traducción Automática

Open searching enables the identification of glycopeptides decorated with previously unknown glycan compositions. Within this article, a streamlined approach for undertaking open searching and subsequent glycan-focused glycopeptide searches are presented for bacterial samples using Acinetobacter baumannii as a model.

Tags

Acinetobacter BaumanniiO-linked GlycopeptidesOpen SearchingGlycosylationProteomeSTB-RPSZIC-HILICChromatographyEnrichmentMass Spectrometry

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Identification of Fatty Acids in Bacillus cereus

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Read Article