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The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
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生物化学
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JoVE 杂志 生物化学
The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

DOI:
10.3791/63242-v

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08:37 min

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November 02, 2021

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, , ,
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,The University of Melbourne

Chapters

  • 00:04Introduction
  • 00:44Processing of Proteome Samples
  • 04:01Liquid Chromatography-Mass Spectrometry (LC-MS) of Proteome/Glycopeptide-Enriched Samples
  • 04:28Analysis of Proteome/Glycopeptide-Enriched Samples
  • 06:08Results: Open Searching and Glycan-Focused Glycopeptide Searches for Acinetobacter baumannii
  • 07:52Conclusion

Summary

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Open searching enables the identification of glycopeptides decorated with previously unknown glycan compositions. Within this article, a streamlined approach for undertaking open searching and subsequent glycan-focused glycopeptide searches are presented for bacterial samples using Acinetobacter baumannii as a model.

Tags

Acinetobacter BaumanniiO-linked GlycopeptidesOpen SearchingGlycosylationProteomeSTB-RPSZIC-HILICChromatographyEnrichmentMass Spectrometry

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