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Protocolo Econômico e Eficiente para Isolar e Culturing Células Dendríticas Derivadas da Medula Óssea de camundongos
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Protocolo Econômico e Eficiente para Isolar e Culturing Células Dendríticas Derivadas da Medula Óssea de camundongos

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04:29 min

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July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

•

04:29 min
July 01, 2022

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38 Views
, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

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Aqui, apresentamos um método econômico e eficiente para isolar e gerar células dendríticas derivadas da medula óssea de camundongos após 7 dias de cultura com 10 ng/mL GM-CSF/IL-4.

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Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

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