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Chapters
- 00:05Introduction
- 00:49Conjugating the 3-Maleimido-PROXYL Nitroxide Spin Label
- 01:38Preparing the NMR Sample for Measuring Intramolecular PRE
- 02:11Setting Up the NMR Spectrometer and Experiment Specific Parameters
- 03:51Setting Up the 1HN–T2 Experiment
- 04:58Making a Diamagnetic Sample by Reducing Spin-Label with Ascorbic Acid
- 05:52Results: 1HN–T2 Rates for Residues 171 – 264 of the Intrinsically Disordered Domain of EWSR1
- 06:30Conclusion
