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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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Bioquímica
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JoVE Revista Bioquímica
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

1,517 Views

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07:24 min

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September 23, 2021

DOI:

10.3791/63057-v

DOI:
10.3791/63057-v

•

07:24 min
September 23, 2021

•

8 Views
,
1Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute,The University of Texas Health Science Center at San Antonio

Summary

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A protocol for the application of paramagnetic relaxation enhancement NMR spectroscopy to detect weak and transient inter- and intra-molecular interactions in intrinsically disordered proteins is presented.

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Johnson, C. N., Libich, D. S. Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins. J. Vis. Exp. (175), e63057, doi:10.3791/63057 (2021).

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