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Automating Aggregate Quantification in Caenorhabditis elegans
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Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

2,355 Views

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07:50 min

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October 14, 2021

DOI:

10.3791/62997-v

DOI:
10.3791/62997-v

•

07:50 min
October 14, 2021

•

4 Views
, , , , ,
1Department of Microbiology and Cell Science,University of Florida, 2Department of Computer and Information Science and Engineering,University of Florida

Summary

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The following protocol describes the development and optimization of a high-throughput workflow for worm culturing, fluorescence imaging, and automated image processing to quantify polyglutamine aggregates as an assessment of changes in proteostasis.

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Cite this Article

Vaziriyan-Sani, A. S., Handy, R. D., Walker, A. C., Pagolu, C. N., Enslow, S. M., Czyż, D. M. Automating Aggregate Quantification in Caenorhabditis elegans. J. Vis. Exp. (176), e62997, doi:10.3791/62997 (2021).

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