Automating Aggregate Quantification in <em>Caenorhabditis elegans</em>

Alfonso S. Vaziriyan-Sani; Robert D. Handy; Alyssa C. Walker; Carol Navya Pagolu; Samantha M. Enslow; Daniel   M. Czy&#380;

doi:10.3791/62997

Journal

/

Biologie

/

Automating Aggregate Quantification in Caenorhabditis elegans

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

2,433 Views

•

07:50 min

•

October 14, 2021

DOI:

10.3791/62997-v

DOI:

10.3791/62997-v

•

07:50 min

October 14, 2021

•

4 Views

Alfonso S. Vaziriyan-Sani¹, Robert D. Handy¹, Alyssa C. Walker¹, Carol Navya Pagolu^1,2, Samantha M. Enslow¹, Daniel M. Czyż¹

¹Department of Microbiology and Cell Science,University of Florida, ²Department of Computer and Information Science and Engineering,University of Florida

Summary

The following protocol describes the development and optimization of a high-throughput workflow for worm culturing, fluorescence imaging, and automated image processing to quantify polyglutamine aggregates as an assessment of changes in proteostasis.

Explore More Videos

Polyglutamine Aggregation Proteostasis Caenorhabditis Elegans Automated Aggregate Counting High-throughput Microscopy Imaging CellProfiler Image Analysis Pipeline Untangled Worms Module Bacterial Genes Neurodegenerative Diseases

Read Article

Automating Aggregate Quantification in Caenorhabditis elegans

Summary

Automatically generated

Explore More Videos