Automating Aggregate Quantification in <em>Caenorhabditis elegans</em>

Alfonso S. Vaziriyan-Sani; Robert D. Handy; Alyssa C. Walker; Carol Navya Pagolu; Samantha M. Enslow; Daniel   M. Czy&#380;

doi:10.3791/62997

ジャーナル

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生物学

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Automating Aggregate Quantification in Caenorhabditis elegans

JoVE Journal
生物学

This content is Free Access.

JoVE Journal 生物学

Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

DOI:

10.3791/62997-v

•

07:50 min

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October 14, 2021

•

Alfonso S. Vaziriyan-Sani, Robert D. Handy, Alyssa C. Walker, Carol Navya Pagolu², Samantha M. Enslow, Daniel M. Czyż

¹Department of Microbiology and Cell Science,University of Florida, ²Department of Computer and Information Science and Engineering,University of Florida

章

00:04Introduction
02:28Image Analysis
05:38Results: The Analysis of Aggregate Detection and the Aggregate Quantification Efficacy Using CellProfiler and Automation

概要

自動翻訳

The following protocol describes the development and optimization of a high-throughput workflow for worm culturing, fluorescence imaging, and automated image processing to quantify polyglutamine aggregates as an assessment of changes in proteostasis.