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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
Chapters
- 00:04Introduction
- 00:53Isolation of Bone Marrow Cells
- 02:14Selection, Differentiation, and Stimulation of BMDMs
- 04:24Results: Comparison of Nitrite and Tetrahydrobiopterin Levels in Media Supplemented with LCM or Recombinant GM-CSF and M-CSF
- 05:54Conclusion
This protocol has been established to culture tetrahydrobiopterin (BH4)- and inducible nitric oxide synthase (iNOS)-deficient primary murine macrophages to study NO-redox biology. The study focuses on reducing potential contamination of BH4 and other artifacts found in traditional isolation and culture methods which may confound experimental outcomes and interpretation of results.
