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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

7,551 Views

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06:34 min

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May 31, 2022

DOI:

10.3791/62834-v

DOI:
10.3791/62834-v

•

06:34 min
May 31, 2022

•

58 Views
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1BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital,University of Oxford, 2Wellcome Centre for Human Genetics,University of Oxford

Summary

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This protocol has been established to culture tetrahydrobiopterin (BH4)- and inducible nitric oxide synthase (iNOS)-deficient primary murine macrophages to study NO-redox biology. The study focuses on reducing potential contamination of BH4 and other artifacts found in traditional isolation and culture methods which may confound experimental outcomes and interpretation of results.

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Cite this Article

Diotallevi, M., Nicol, T., Ayaz, F., Bailey, J., Shaw, A., McNeill, E., Davies, B., Channon, K. M., Crabtree, M. J. Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology. J. Vis. Exp. (183), e62834, doi:10.3791/62834 (2022).

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