This content is Free Access.
Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
7,464 Views
•
06:34 min
•
May 31, 2022
DOI:
概要
Automatically generated
This protocol has been established to culture tetrahydrobiopterin (BH4)- and inducible nitric oxide synthase (iNOS)-deficient primary murine macrophages to study NO-redox biology. The study focuses on reducing potential contamination of BH4 and other artifacts found in traditional isolation and culture methods which may confound experimental outcomes and interpretation of results.
Read Article
Cite this Article
Diotallevi, M., Nicol, T., Ayaz, F., Bailey, J., Shaw, A., McNeill, E., Davies, B., Channon, K. M., Crabtree, M. J. Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology. J. Vis. Exp. (183), e62834, doi:10.3791/62834 (2022).
Copy