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Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
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Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

DOI:
10.3791/62537-v

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09:01 min

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May 23, 2021

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, , , ,
1Department of Pharmacology & Physiology,Drexel University College of Medicine

Chapters

  • 00:04Introduction
  • 00:57Uptake of sEVs by Neuro-2a Cells
  • 01:38Primary Astrocytic Cultures
  • 03:34Immunofluorescence
  • 05:03In Vivo Uptake of sEVs and Immunohistochemistry
  • 06:43Results: Uptake of Fluorescent Labeled Small Extracellular Vesicles
  • 08:11Conclusion

Summary

자동 번역

자동 번역

We describe a protocol to label macrophage-derived small extracellular vesicles with PKH dyes and observe their uptake in vitro and in the spinal cord after intrathecal delivery.

Tags

Fluorescent Labeled Small Extracellular VesiclesSEVs UptakeSpinal CordIn VitroCell VisualizationNeuro-2a CellsPostnatal PupsCortical LobesDissociationAstrocytesMicrogliaOligodendrocytesMechanistic StudiesTherapeutic StudiesSpinal DisordersPainInflammation

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