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Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
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神经科学
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JoVE 杂志 神经科学
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

3,271 Views

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09:01 min

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May 23, 2021

DOI:

10.3791/62537-v

DOI:
10.3791/62537-v

•

09:01 min
May 23, 2021

•

2 Views
, , , ,
1Department of Pharmacology & Physiology,Drexel University College of Medicine

Summary

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We describe a protocol to label macrophage-derived small extracellular vesicles with PKH dyes and observe their uptake in vitro and in the spinal cord after intrathecal delivery.

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Gupta, R., Luo, X., Lin, Z., Tian, Y., Ajit, S. K. Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord. J. Vis. Exp. (171), e62537, doi:10.3791/62537 (2021).

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