We describe a protocol to label macrophage-derived small extracellular vesicles with PKH dyes and observe their uptake in vitro and in the spinal cord after intrathecal delivery.
Gupta, R., Luo, X., Lin, Z., Tian, Y., Ajit, S. K. Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord. J. Vis. Exp. (171), e62537, doi:10.3791/62537 (2021).