Rivista
/
Neuroscienze
/
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

3,271 Views

•

09:01 min

•

May 23, 2021

DOI:

10.3791/62537-v

DOI:
10.3791/62537-v

•

09:01 min
May 23, 2021

•

2 Views
, , , ,
1Department of Pharmacology & Physiology,Drexel University College of Medicine

Summary

Automatically generated

We describe a protocol to label macrophage-derived small extracellular vesicles with PKH dyes and observe their uptake in vitro and in the spinal cord after intrathecal delivery.

Explore More Videos

Fluorescent Labeled Small Extracellular VesiclesSEVs UptakeSpinal CordIn VitroCell VisualizationNeuro-2a CellsPostnatal PupsCortical LobesDissociationAstrocytesMicrogliaOligodendrocytesMechanistic StudiesTherapeutic StudiesSpinal DisordersPainInflammation

Read Article

Cite this Article

Gupta, R., Luo, X., Lin, Z., Tian, Y., Ajit, S. K. Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord. J. Vis. Exp. (171), e62537, doi:10.3791/62537 (2021).

Download .ris file

Copy

Share Video

.

Copy