The increasing demand for large-scale data collection in cryogenic electron tomography requires high-throughput image acquisition routines. Described here is a protocol that implements the recent developments of advanced acquisition strategies aimed at maximizing the time-efficiency and throughput of tomographic data collection.
Weis, F., Hagen, W. J. H., Schorb, M., Mattei, S. Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition. J. Vis. Exp. (169), e62383, doi:10.3791/62383 (2021).