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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

DOI:
10.3791/62383-v

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08:16 min

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March 19, 2021

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, , ,
1Structural and Computational Biology Unit,European Molecular Biology Laboratory, 2Electron Microscopy Core Facility,European Molecular Biology Laboratory, 3Imaging Centre,European Molecular Biology Laboratory

'Bölümler'

  • 00:05Introduction
  • 00:38Selecting the Targets
  • 01:31Generate Virtual Maps
  • 02:07Microscope Tuning
  • 02:43Set up Navigator
  • 04:05Set the Focus/Track Positions
  • 05:00Set up Additional Scripts
  • 05:27Run
  • 06:19Results: Optimization of Cryogenic Electron Tomography Data Acquisition
  • 07:46Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

The increasing demand for large-scale data collection in cryogenic electron tomography requires high-throughput image acquisition routines. Described here is a protocol that implements the recent developments of advanced acquisition strategies aimed at maximizing the time-efficiency and throughput of tomographic data collection.

'Etiketler'

Cryogenic Electron TomographyData AcquisitionSerialEMVirtual Anchor MapsComa-free AlignmentAstigmatism CorrectionTilt Series AcquisitionPreview MapsFocus/track Distance

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