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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
Capítulos
- 00:05Introduction
- 00:38Selecting the Targets
- 01:31Generate Virtual Maps
- 02:07Microscope Tuning
- 02:43Set up Navigator
- 04:05Set the Focus/Track Positions
- 05:00Set up Additional Scripts
- 05:27Run
- 06:19Results: Optimization of Cryogenic Electron Tomography Data Acquisition
- 07:46Conclusion
The increasing demand for large-scale data collection in cryogenic electron tomography requires high-throughput image acquisition routines. Described here is a protocol that implements the recent developments of advanced acquisition strategies aimed at maximizing the time-efficiency and throughput of tomographic data collection.
