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Biochemistry

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JoVE Journal Biochemistry
Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
 

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Article DOI: 10.3791/62378-v • 07:12 min • July 5th, 2021
July 5th, 2021 •
*1, *1, 1, 1
1Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
* These authors contributed equally

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요약
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This article provides step-by-step instructions for making fully-corrected accurate FRET measurements on individual, freely diffusing biomolecules using the open-source, inexpensive smfBox, from switch on, through alignment and focusing, to data collection and analysis.

Tags

Single-molecule FRET Open-source SmfBox Biomolecules SmFRET Conformations Protein Folding Protein DNA Interactions DNA Allostery Laser Control Center SmOTTER Acquisition Software Cy3B Dye Laser Duty Cycles Panel ALEX Period Z Focus Ta
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