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Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
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Biochemie
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JoVE Journal Biochemie
Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

3,131 Views

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07:12 min

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July 05, 2021

DOI:

10.3791/62378-v

DOI:
10.3791/62378-v

•

07:12 min
July 05, 2021

•

13 Views
, , ,
1Sheffield Institute for Nucleic Acids, Department of Chemistry,University of Sheffield

Summary

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This article provides step-by-step instructions for making fully-corrected accurate FRET measurements on individual, freely diffusing biomolecules using the open-source, inexpensive smfBox, from switch on, through alignment and focusing, to data collection and analysis.

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Cite this Article

Abdelhamid, M. A. S., Rhind-Tutt, A. V., Ambrose, B., Craggs, T. D. Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox. J. Vis. Exp. (173), e62378, doi:10.3791/62378 (2021).

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