Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Mahmoud A. S. Abdelhamid; Alice V. Rhind-Tutt; Benjamin Ambrose; Timothy D. Craggs

doi:10.3791/62378

Journal

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Biochimie

/

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Journal JoVE
Biochimie

This content is Free Access.

Journal JoVE Biochimie

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

3,131 Views

•

07:12 min

•

July 05, 2021

DOI:

10.3791/62378-v

DOI:

10.3791/62378-v

•

07:12 min

July 05, 2021

•

13 Views

Mahmoud A. S. Abdelhamid*¹, Alice V. Rhind-Tutt*¹, Benjamin Ambrose¹, Timothy D. Craggs¹

¹Sheffield Institute for Nucleic Acids, Department of Chemistry,University of Sheffield

Summary

This article provides step-by-step instructions for making fully-corrected accurate FRET measurements on individual, freely diffusing biomolecules using the open-source, inexpensive smfBox, from switch on, through alignment and focusing, to data collection and analysis.

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Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

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