JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
36,751 Views
•
08:06 min
•
February 15, 2021
DOI:
1The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 2Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, 3ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 4Headquarter, Walter Reed Army Institute of Research
Summary
Automatically generated
We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.
Read Article
Cite this Article
Meyerhoff, J., Muhie, S., Chakraborty, N., Naidu, L., Sowe, B., Hammamieh, R., Jett, M., Gautam, A. Microdissection of Mouse Brain into Functionally and Anatomically Different Regions. J. Vis. Exp. (168), e61941, doi:10.3791/61941 (2021).
Copy