We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.
Meyerhoff, J., Muhie, S., Chakraborty, N., Naidu, L., Sowe, B., Hammamieh, R., Jett, M., Gautam, A. Microdissection of Mouse Brain into Functionally and Anatomically Different Regions. J. Vis. Exp. (168), e61941, doi:10.3791/61941 (2021).