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Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
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Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
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08:06 min
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February 15, 2021
DOI:
1The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 2Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, 3ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 4Headquarter, Walter Reed Army Institute of Research
Summary
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We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.
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Meyerhoff, J., Muhie, S., Chakraborty, N., Naidu, L., Sowe, B., Hammamieh, R., Jett, M., Gautam, A. Microdissection of Mouse Brain into Functionally and Anatomically Different Regions. J. Vis. Exp. (168), e61941, doi:10.3791/61941 (2021).
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