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Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
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Neuroscienze
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JoVE Journal Neuroscienze
Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

DOI:
10.3791/61941-v

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08:06 min

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February 15, 2021

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, , , , , , ,
1The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 2Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, 3ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 4Headquarter, Walter Reed Army Institute of Research

Capitoli

  • 00:04Introduction
  • 01:41Mouse Brain Removal
  • 03:26Mouse Brain Dissection
  • 06:40Results: Mouse Brain Removal and Dissection
  • 07:22Conclusion

Summary

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We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.

Tags

Mouse BrainBrain DissectionBrain RegionsMolecular AnalysisAnatomical RegionsStructural LandmarksMicrodissectionNeuroscienzeBrain Anatomy

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