Journal
/
신경과학
/
A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model

A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model

DOI:
10.3791/61740-v

•

09:42 min

•

June 04, 2021

•

, ,
1Department of Anesthesiology, Taipei Veterans General Hospital,National Yang-Ming University School of Medicine, 2Department of Neuroscience, Center for Brain Immunology and Glia (BIG),University of Virginia School of Medicine

Chapters

  • 00:04Introduction
  • 01:19Ligation of the Ipsilateral Common Carotid Artery
  • 02:35Skull Thinning Above the Middle Cranial Artery Branch and Photoactivation
  • 04:44Intravital Imaging and tPA Administration
  • 06:34Results: Immunofluorescence Labeling, Intravital Imaging, and the Effects of tPA Treatment on CBF Recovery and Infarct Size
  • 08:50Conclusion

Summary

자동 번역

자동 번역

Traditional photothrombotic stroke (PTS) models mainly induce dense platelet aggregates of a high resistance to tissue plasminogen activator (tPA)-lytic treatment. Here a modified murine PTS model is introduced by co-injecting thrombin and photosensitive dye for photoactivation. The thrombin-enhanced PTS model produces mixed platelet:fibrin clots and is highly sensitive to tPA-thrombolysis.

Tags

Keywords: Photothrombotic Stroke ModelFibrin-enrichedTPA-sensitiveThrombolytic TherapyBlood ClotAcute Stroke PatientPreclinical Stroke ResearchThrombolytic Therapies

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/61740?article_type=v

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
check_url/kr/61740?article_type=v

Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain
check_url/kr/61740?article_type=v

Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
check_url/kr/61740?article_type=v

Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning
check_url/kr/61740?article_type=v

Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells
check_url/kr/61740?article_type=v

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
check_url/kr/61740?article_type=v

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke
check_url/kr/61740?article_type=v

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats
check_url/kr/61740?article_type=v

Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons
check_url/kr/61740?article_type=v

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

Read Article