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A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model

A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model

DOI:
10.3791/61740-v

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09:42 min

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June 04, 2021

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, ,
1Department of Anesthesiology, Taipei Veterans General Hospital,National Yang-Ming University School of Medicine, 2Department of Neuroscience, Center for Brain Immunology and Glia (BIG),University of Virginia School of Medicine

Chapters

  • 00:04Introduction
  • 01:19Ligation of the Ipsilateral Common Carotid Artery
  • 02:35Skull Thinning Above the Middle Cranial Artery Branch and Photoactivation
  • 04:44Intravital Imaging and tPA Administration
  • 06:34Results: Immunofluorescence Labeling, Intravital Imaging, and the Effects of tPA Treatment on CBF Recovery and Infarct Size
  • 08:50Conclusion

Summary

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Traditional photothrombotic stroke (PTS) models mainly induce dense platelet aggregates of a high resistance to tissue plasminogen activator (tPA)-lytic treatment. Here a modified murine PTS model is introduced by co-injecting thrombin and photosensitive dye for photoactivation. The thrombin-enhanced PTS model produces mixed platelet:fibrin clots and is highly sensitive to tPA-thrombolysis.

Tags

Keywords: Photothrombotic Stroke ModelFibrin-enrichedTPA-sensitiveThrombolytic TherapyBlood ClotAcute Stroke PatientPreclinical Stroke ResearchThrombolytic Therapies

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