Journal
/
생화학
/
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

5,750 Views

•

07:31 min

•

July 16, 2020

DOI:

10.3791/61298-v

DOI:
10.3791/61298-v

•

07:31 min
July 16, 2020

•

6 Views
, , , ,
1Department of Medicinal Chemistry, School of Pharmacy,Virginia Commonwealth University, 2Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy,Virginia Commonwealth University

Summary

Automatically generated

Presented here is a protocol for the determination of oligomeric state of membrane proteins that utilizes a native cell membrane nanoparticle system in conjunction with electron microscopy.

Explore More Videos

Native Cell Membrane NanoparticlesMembrane Protein-protein Interaction AnalysisElectron MicroscopyMembrane Protein StructureNative EnvironmentHigh-resolution Structure DeterminationMembrane Active PolymerNickel NTA ColumnFast Protein Liquid ChromatographyNegative Stain Grids

Read Article

Cite this Article

Kroeck, K. G., Qiu, W., Catalano, C., Trinh, T. K. H., Guo, Y. Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis. J. Vis. Exp. (161), e61298, doi:10.3791/61298 (2020).

Download .ris file

Copy

Share Video

.

Copy