Journal
/
Biochemie
/
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

5,750 Views

•

07:31 min

•

July 16, 2020

DOI:

10.3791/61298-v

DOI:
10.3791/61298-v

•

07:31 min
July 16, 2020

•

6 Views
, , , ,
1Department of Medicinal Chemistry, School of Pharmacy,Virginia Commonwealth University, 2Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy,Virginia Commonwealth University

Summary

Automatically generated

Presented here is a protocol for the determination of oligomeric state of membrane proteins that utilizes a native cell membrane nanoparticle system in conjunction with electron microscopy.

Explore More Videos

Native Cell Membrane NanoparticlesMembrane Protein-protein Interaction AnalysisElectron MicroscopyMembrane Protein StructureNative EnvironmentHigh-resolution Structure DeterminationMembrane Active PolymerNickel NTA ColumnFast Protein Liquid ChromatographyNegative Stain Grids

Read Article

Cite this Article

Kroeck, K. G., Qiu, W., Catalano, C., Trinh, T. K. H., Guo, Y. Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis. J. Vis. Exp. (161), e61298, doi:10.3791/61298 (2020).

Download .ris file

Copy

Share Video

.

Copy