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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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JoVE Journal Biochimica
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

5,750 Views

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07:31 min

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July 16, 2020

DOI:

10.3791/61298-v

DOI:
10.3791/61298-v

•

07:31 min
July 16, 2020

•

6 Views
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1Department of Medicinal Chemistry, School of Pharmacy,Virginia Commonwealth University, 2Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy,Virginia Commonwealth University

Summary

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Presented here is a protocol for the determination of oligomeric state of membrane proteins that utilizes a native cell membrane nanoparticle system in conjunction with electron microscopy.

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Cite this Article

Kroeck, K. G., Qiu, W., Catalano, C., Trinh, T. K. H., Guo, Y. Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis. J. Vis. Exp. (161), e61298, doi:10.3791/61298 (2020).

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