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CXCR4在Murine B-1a细胞上的逆转录病毒过度表达和靶向B-1a细胞向骨髓和IgM生产转移的采用转移
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
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CXCR4在Murine B-1a细胞上的逆转录病毒过度表达和靶向B-1a细胞向骨髓和IgM生产转移的采用转移

DOI:
10.3791/61003-v

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08:22 min

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May 31, 2020

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, , , ,
1Department of Microbiology, Immunology, Cancer Biology,University of Virginia, 2Cardiovascular Research Center,University of Virginia, 3Beirne B. Carter Center for Immunology Research,University of Virginia

Chapters

  • 00:04Introduction
  • 01:02Peritoneal B-1 Cell Magnetic Separation and Enrichment
  • 04:13Peritoneal B-1 Cell Stimulation
  • 04:58Retroviral Transduction
  • 05:51Results: Representative Peritoneal B-1 Cell Enrichment, Transduction, Characterization, and Quantification
  • 07:54Conclusion

Summary

자동 번역

자동 번역

在这里，我们描述了一种抗逆转录病毒过度表达和采用迁移的鼠B-1a细胞的方法，以检查体内B-1a细胞的迁移和定位。该协议可扩展到不同的下游功能检测，包括定量供体B-1a细胞定位或分析供体细胞衍生分泌因子的收养后转移。

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AI-powered Writing AssistantCXCR4 OverexpressionB-1a Cell MigrationIgM ProductionPeritoneal Fluid HarvestingAdoptive TransferGene DeliveryIn Vivo Cell Processes

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