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Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

DOI:
10.3791/61003-v

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08:22 min

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May 31, 2020

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, , , ,
1Department of Microbiology, Immunology, Cancer Biology,University of Virginia, 2Cardiovascular Research Center,University of Virginia, 3Beirne B. Carter Center for Immunology Research,University of Virginia

Capitoli

  • 00:04Introduction
  • 01:02Peritoneal B-1 Cell Magnetic Separation and Enrichment
  • 04:13Peritoneal B-1 Cell Stimulation
  • 04:58Retroviral Transduction
  • 05:51Results: Representative Peritoneal B-1 Cell Enrichment, Transduction, Characterization, and Quantification
  • 07:54Conclusion

Summary

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Here we describe a method for retroviral overexpression and adoptive transfer of murine B-1a cells to examine in vivo B-1a cell migration and localization. This protocol can be extended for diverse downstream functional assays including quantification of donor B-1a cell localization or analysis of donor cell-derived secreted factors post-adoptive transfer.

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AI-powered Writing AssistantCXCR4 OverexpressionB-1a Cell MigrationIgM ProductionPeritoneal Fluid HarvestingAdoptive TransferGene DeliveryIn Vivo Cell Processes

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