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High-Frequency Ultrasound Echocardiography om zebravissen cardiale functie te beoordelen
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High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
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High-Frequency Ultrasound Echocardiography om zebravissen cardiale functie te beoordelen

8,159 Views

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08:34 min

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March 12, 2020

DOI:

10.3791/60976-v

DOI:
10.3791/60976-v

•

08:34 min
March 12, 2020

•

26 Views
, , , , , , ,
1Stanford Cardiovascular Institute,Stanford University, 2Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, Harvard Medical School

Summary

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We beschrijven een protocol om hartmorfologie en functie bij volwassen zebravissen te beoordelen met behulp van hoogfrequente echocardiografie. De methode maakt visualisatie van het hart en de daaropvolgende kwantificering van functionele parameters mogelijk, zoals hartslag (HR), hartoutput (CO), fractionele gebiedsverandering (FAC), uitwerpfractie (EF) en bloedinstroom en uitstroomsnelheden.

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Cite this Article

Evangelisti, A., Schimmel, K., Joshi, S., Shah, K., Fisch, S., Alexander, K. M., Liao, R., Morgado, I. High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function. J. Vis. Exp. (157), e60976, doi:10.3791/60976 (2020).

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