High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

Alessandro Evangelisti; Katharina Schimmel; Shaurya Joshi; Kavya Shah; Sudeshna Fisch; Kevin  M. Alexander; Ronglih Liao; Isabel Morgado

doi:10.3791/60976

ジャーナル

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発生生物学

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High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

8,214 Views

•

08:34 min

•

March 12, 2020

DOI:

10.3791/60976-v

DOI:

10.3791/60976-v

•

08:34 min

March 12, 2020

•

26 Views

Alessandro Evangelisti¹, Katharina Schimmel¹, Shaurya Joshi², Kavya Shah¹, Sudeshna Fisch², Kevin M. Alexander¹, Ronglih Liao¹, Isabel Morgado¹

¹Stanford Cardiovascular Institute,Stanford University, ²Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, Harvard Medical School

概要

We describe a protocol to assess heart morphology and function in adult zebrafish using high-frequency echocardiography. The method allows visualization of the heart and subsequent quantification of functional parameters, such as heart rate (HR), cardiac output (CO), fractional area change (FAC), ejection fraction (EF), and blood inflow and outflow velocities.

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High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

概要

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