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发育生物学
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High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
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发育生物学
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JoVE 杂志 发育生物学
High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

8,159 Views

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08:34 min

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March 12, 2020

DOI:

10.3791/60976-v

DOI:
10.3791/60976-v

•

08:34 min
March 12, 2020

•

26 Views
, , , , , , ,
1Stanford Cardiovascular Institute,Stanford University, 2Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, Harvard Medical School

Summary

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We describe a protocol to assess heart morphology and function in adult zebrafish using high-frequency echocardiography. The method allows visualization of the heart and subsequent quantification of functional parameters, such as heart rate (HR), cardiac output (CO), fractional area change (FAC), ejection fraction (EF), and blood inflow and outflow velocities.

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Cite this Article

Evangelisti, A., Schimmel, K., Joshi, S., Shah, K., Fisch, S., Alexander, K. M., Liao, R., Morgado, I. High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function. J. Vis. Exp. (157), e60976, doi:10.3791/60976 (2020).

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