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基于AFM的力光谱单分子共价附价
JoVE 신문
화학
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JoVE 신문 화학
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
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基于AFM的力光谱单分子共价附价

DOI:
10.3791/60934-v

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10:37 min

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March 16, 2020

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, , , ,
1Institute of Physical Chemistry,Albert-Ludwigs-Universität Freiburg, 2Chair in Polymer Chemistry,Saarland University, 3Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg

Chapters

  • 00:04Introduction
  • 00:52Surface Activation, Silanization, and PEGylation
  • 03:03Covalent Polymer Attachment
  • 04:21Determining the Inverse Optical Lever Sensitivity (InVOLS)
  • 06:13Determining the AFM Cantilever Force Constant
  • 07:06Data Collection
  • 08:10Results: Single-Molecule Stretching and Desorption
  • 09:44Conclusion

Summary

자동 번역

자동 번역

探针分子附于原子力显微镜（AFM）悬臂尖端是研究其物理特性的关键技术。这使我们能够通过基于 AFM 的单分子力光谱，确定聚合物的拉伸力、解吸力和长度，具有高可重复性。

Tags

Single Molecule Force SpectroscopyAFM-based Force SpectroscopyCovalent AttachmentPolymer BindingSilane-PEG-malPlasma ActivationToluenePEGPolystyrenePolynipamCapillary ForcesControl Samples

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