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Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
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Chemistry
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JoVE Journal Chemistry
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

English

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9,232 Views

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10:37 min

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March 16, 2020

DOI:

10.3791/60934-v

DOI:
10.3791/60934-v

•

10:37 min
March 16, 2020

•

4 Views
, , , ,
1Institute of Physical Chemistry,Albert-Ludwigs-Universität Freiburg, 2Chair in Polymer Chemistry,Saarland University, 3Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg

Summary

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Covalent attachment of probe molecules to atomic force microscopy (AFM) cantilever tips is an essential technique for the investigation of their physical properties. This allows us to determine the stretching force, desorption force and length of polymers via AFM-based single molecule force spectroscopy with high reproducibility.

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Cite this Article

Kolberg, A., Wenzel, C., Hugel, T., Gallei, M., Balzer, B. N. Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy. J. Vis. Exp. (157), e60934, doi:10.3791/60934 (2020).

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