Rivista
/
Chimica
/
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
JoVE Journal
Chimica
This content is Free Access.
JoVE Journal Chimica
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

9,232 Views

•

10:37 min

•

March 16, 2020

DOI:

10.3791/60934-v

DOI:
10.3791/60934-v

•

10:37 min
March 16, 2020

•

4 Views
, , , ,
1Institute of Physical Chemistry,Albert-Ludwigs-Universität Freiburg, 2Chair in Polymer Chemistry,Saarland University, 3Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg

Summary

Automatically generated

Covalent attachment of probe molecules to atomic force microscopy (AFM) cantilever tips is an essential technique for the investigation of their physical properties. This allows us to determine the stretching force, desorption force and length of polymers via AFM-based single molecule force spectroscopy with high reproducibility.

Explore More Videos

Single Molecule Force SpectroscopyAFM-based Force SpectroscopyCovalent AttachmentPolymer BindingSilane-PEG-malPlasma ActivationToluenePEGPolystyrenePolynipamCapillary ForcesControl Samples

Read Article

Cite this Article

Kolberg, A., Wenzel, C., Hugel, T., Gallei, M., Balzer, B. N. Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy. J. Vis. Exp. (157), e60934, doi:10.3791/60934 (2020).

Download .ris file

Copy

Share Video

.

Copy