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Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
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Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

11,481 Views

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09:41 min

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May 14, 2020

DOI:

10.3791/60919-v

DOI:
10.3791/60919-v

•

09:41 min
May 14, 2020

•

8 Views
, ,
1Department of Plant and Microbial Biology,University of Zurich, 2Biophore – UNIL, 3Laboratory of Cell and Molecular Biology, Institute of Biology,University of Neuchâtel

Summary

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Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.

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Ribosome Affinity Purification (TRAP)Arabidopsis ThalianaRoot DevelopmentCell Type-specificPolysome RNALow-input RNALateral Root FormationCell-cell CommunicationCell WallMechanical SignalingSeed SterilizationExogenous TreatmentNAA

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Cite this Article

Thellmann, M., Andersen, T. G., Vermeer, J. E. Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale. J. Vis. Exp. (159), e60919, doi:10.3791/60919 (2020).

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