Translating Ribosome Affinity Purification (TRAP) to Investigate <em>Arabidopsis thaliana</em> Root Development at a Cell Type-Specific Scale

Martha Thellmann; Tonni  Grube Andersen; Joop  EM Vermeer

doi:10.3791/60919

/

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

JoVE 杂志
发育生物学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志发育生物学

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

11,481 Views

•

09:41 min

•

May 14, 2020

DOI:

10.3791/60919-v

DOI:

10.3791/60919-v

•

09:41 min

May 14, 2020

•

8 Views

Martha Thellmann¹, Tonni Grube Andersen², Joop EM Vermeer^1,3

¹Department of Plant and Microbial Biology,University of Zurich, ²Biophore – UNIL, ³Laboratory of Cell and Molecular Biology, Institute of Biology,University of Neuchâtel

Summary

Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.

Explore More Videos

Ribosome Affinity Purification (TRAP)Arabidopsis Thaliana Root Development Cell Type-specific Polysome RNA Low-input RNA Lateral Root Formation Cell-cell Communication Cell Wall Mechanical Signaling Seed Sterilization Exogenous Treatment NAA

Read Article

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Summary

Automatically generated

Explore More Videos