Translating Ribosome Affinity Purification (TRAP) to Investigate <em>Arabidopsis thaliana</em> Root Development at a Cell Type-Specific Scale

Martha Thellmann; Tonni  Grube Andersen; Joop  EM Vermeer

doi:10.3791/60919

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Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

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Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

11,453 Views

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09:41 min

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May 14, 2020

DOI:

10.3791/60919-v

DOI:

10.3791/60919-v

•

09:41 min

May 14, 2020

•

8 Views

Martha Thellmann¹, Tonni Grube Andersen², Joop EM Vermeer^1,3

¹Department of Plant and Microbial Biology,University of Zurich, ²Biophore – UNIL, ³Laboratory of Cell and Molecular Biology, Institute of Biology,University of Neuchâtel

Summary

Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.

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Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Summary

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